cDNA Synthesis (complementary DNA synthesis) is a molecular biology technique used to convert messenger RNA (mRNA) into complementary DNA (cDNA) using the enzyme reverse transcriptase. This process is essential in studying gene expression, creating cDNA libraries, and performing techniques such as RT-PCR and qPCR.
RNA Extraction by TRIzol™ Reagent is a widely used method for isolating high-quality total RNA from various biological samples, such as cells, tissues, or microorganisms. TRIzol™ (also known as TRI Reagent® or phenol-guanidine-based reagent) combines phenol and guanidine isothiocyanate to lyse cells and inactivate RNases during the extraction process.
Refer to laboratory methods used to detect and analyze the binding or
hybridization of nucleic acids, such as DNA or RNA. These techniques allow
scientists to study and manipulate genetic material and provide valuable
information about gene expression, genetic variations, and interactions between
nucleic acids.
Agarose gel electrophoresis is a fundamental technique in molecular biology with a wide range of applications. It is primarily used to separate and analyze nucleic acids (DNA and RNA) based on their size.
The Polymerase Chain Reaction (PCR) is a powerful laboratory technique used
to amplify specific DNA sequences, allowing scientists to produce millions or even
billions of copies of a target DNA fragment in just a few hours. The Polymerase
Chain Reaction (PCR) was not a discovery, but rather an invention by Kary Mullis
in 1983.
DNA Extraction
The first isolation of deoxyribonucleic acid (DNA) was done in 1869 by Friedrich
Miescher . DNA extraction is the process of isolating DNA from the cells of an
organism isolated from a sample, typically a biological sample
Molecular biology is emerging as one of the potential Life Science courses, which have wide applications